Search for the modification-restriction systems in Agrobacteria DNA
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15 صفحه اولDNA restriction-modification systems mediate plasmid maintenance.
Two plasmid-carried restriction-modification (R-M) systems, EcoRI (from pMB1 of Escherichia coli) and Bsp6I (from pXH13 of Bacillus sp. strain RFL6), enhance plasmid segregational stability in E. coli and Bacillus subtilis, respectively. Inactivation of the endonuclease or the presence of the methylase in trans abolish the stabilizing activity of the R-M systems. We propose that R-M systems med...
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Since the role of DNA restriction-modification (R-M) systems of hsd genes has been elucidated, recipient cells for unmodified or foreign DNA are usually restrictionless mutants. Such mutants may either modify and have the phenotype rm+ or fail to modify and have the phenotype rm-. Each R-M system consists of three genes: hsdS for specificity, hsdR for restriction, and hsdM for modification (1)....
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 1987
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.0001df